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Timentin溶液(100mg/ml)

Timentin Solution（100mg/ml）

● 产品包装：

CAS: 4697-14-7/61177-45-5(替卡西林钠/克拉维酸钾)

Formula: C₁₅H₁₄N₂Na₂O₆S₂/C₈H₈KNO₅

Appearance: pale yellow clear Solution

Typical Working Concentration: 50-200 mg/L

● 产品简介：

Timentin is a suitable antibiotic for Agrobacterium counterselection and a mixture of two antibiotics: ticarcillin and clavulanic acid. Most wild Agrobacterium isolates have beta-lactamase activity which clavulanic acid exhibits activity towards. Many Agrobacterium isolates are highly susceptible to Timentin. Agrobacterium strains carrying the standard pBR beta-lactamase gene are also Timentin (clavulanic acid) susceptible. Timentin killing of Agrobacterium wild-type strains is three logs greater than with comparable doses of carbenicillin.

In plant transformation experiments, 100 μg/ml Timentin is sufficient to counterselect Agrobacterium. To improve efficacy (of any antimicrobial), solid media are best slightly dried before use. At these concentrations, Timentin exhibits no phytotoxicity to Arabidopsis root cultures.

● 保存条件：

   -20℃保存。有效期12个月。

● References:

1 Zimmerman TW (1995) Effect of Timentin for controlling Agrobacterium tumefaciens following cocultivation on select plant species. In Vitro Cell Dev Biol 31:70A

2 Cheng Z M et al. Timentin as an alternative antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation. Plant Cell Reports (1998) 17: 646–649